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發(fā)布時(shí)間：2018/11/2
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Capricorn Scientific成立于2013年，是一家新的德國細(xì)胞培養(yǎng)基，血清和試劑生產(chǎn)商。我們生產(chǎn)的主要重點(diǎn)是生產(chǎn)用于生物技術(shù)，診斷和研究應(yīng)用的高質(zhì)量動(dòng)物血清。嚴(yán)格的測(cè)試程序以及全面的可追溯性記錄和文檔保證了我們產(chǎn)品的和可重復(fù)的質(zhì)量。
作為一家規(guī)模較小的公司，我們可以靈活地提供各種傳統(tǒng)和定制產(chǎn)品，以更低的成本，更短的交付周期和個(gè)性化的客戶服務(wù)滿足當(dāng)今市場不斷增長的需求。
我們的技術(shù)，經(jīng)驗(yàn)豐富的專家團(tuán)隊(duì)多年來一直致力于細(xì)胞培養(yǎng)領(lǐng)域。我們致力于為活躍的細(xì)胞培養(yǎng)公司提供高質(zhì)量，可靠性和服務(wù)。
Capricorn FM1-F說明書
冷凍一種含胎牛血清的細(xì)胞保藏培養(yǎng)基
貓編號(hào)：FM1-F(50毫升)
產(chǎn)品闡述，產(chǎn)品說明
凍干粉是一種含有10%DMSO和20%FBS的冷凍保護(hù)介質(zhì)。所用的血清在細(xì)胞保存期間被廣泛測(cè)試以保護(hù)細(xì)胞。
申請(qǐng)
-冷凍保存多種高活力的細(xì)胞類型
-現(xiàn)成的解決方案
商品概述
不育試驗(yàn)
貯存≤-15°C.凍干粉是一種光敏溶液.在運(yùn)輸和儲(chǔ)存期間，應(yīng)該保護(hù)它不受光線的影響。
凍結(jié)議定書
在冷凍保存前，應(yīng)檢查細(xì)胞是否受到污染。FreezeMe可以與任何標(biāo)準(zhǔn)的冷凍協(xié)議一起使用。
懸浮培養(yǎng)物的低溫保存
-計(jì)算冷凍保存的活細(xì)胞數(shù)。細(xì)胞應(yīng)處于生長中期。離心5 min至顆粒細(xì)胞(200~400 g)。把上清液移到小的 在不打擾細(xì)胞的情況下。
-預(yù)冷(4°C~8°C)的復(fù)蘇細(xì)胞(5x106~107細(xì)胞/ml)。
-進(jìn)入低溫儲(chǔ)存瓶。將小瓶置于4°C，5 min內(nèi)開始冷凍。細(xì)胞以1°C/min的速度緩慢冷凍(可編程冷卻器或?qū)⑿∑糠湃雿u葉) 在攝氏-70度至攝氏-90度的冷凍機(jī)內(nèi)放置箱)。
-然后將儲(chǔ)存瓶轉(zhuǎn)移到液氮儲(chǔ)存。
貼壁培養(yǎng)的冷凍保存
-用溫和的解離劑將細(xì)胞與底物分離。特別是敏感細(xì)胞使用酸性磷酸酶(CAT)。否避免細(xì)胞損傷。必要時(shí)滅活解離劑。
-在完整的培養(yǎng)基中復(fù)蘇分離的細(xì)胞，并建立可行的細(xì)胞計(jì)數(shù)。
離心5 min至顆粒細(xì)胞(200~400 g)。將上清液移至小體積，而不干擾細(xì)胞。
-預(yù)冷(4°C~8°C)的復(fù)蘇細(xì)胞(5x106~107細(xì)胞/ml)。
-進(jìn)入低溫儲(chǔ)存瓶。將小瓶置于4°C，5 min內(nèi)開始冷凍。細(xì)胞以1°C/min的速度緩慢冷凍(可編程冷卻器或?qū)⑿∑糠湃雿u葉) 在攝氏-70度至攝氏-90度的冷凍機(jī)內(nèi)放置箱)。
-然后將儲(chǔ)存瓶轉(zhuǎn)移到液氮儲(chǔ)存。
凍存細(xì)胞可通過以下程序解凍
離心法，離心過濾
-從儲(chǔ)存中取出細(xì)胞，在37°C水浴中迅速解凍。摩羯座科學(xué)推薦使用經(jīng)批準(zhǔn)的安全護(hù)目鏡來保護(hù)眼睛。我們還建議使用安全手套來保護(hù) 裸露的皮膚。-將1至2毫升的解凍細(xì)胞放入25毫升的*培養(yǎng)基中。輕輕混合細(xì)胞懸液。
-80 g離心2~3 min。
-檢查細(xì)胞顆粒的上清和可見度。在不干擾細(xì)胞的情況下丟棄上清液。
-輕輕地將細(xì)胞重新懸浮在完整的培養(yǎng)基中，并進(jìn)行可行的細(xì)胞計(jì)數(shù)。
-打平細(xì)胞。細(xì)胞接種量應(yīng)至少為3x105個(gè)活細(xì)胞/ml。
直接電鍍
-從儲(chǔ)存中取出細(xì)胞，在37°C水浴中迅速解凍。摩羯座科學(xué)推薦使用經(jīng)批準(zhǔn)的安全護(hù)目鏡來保護(hù)眼睛。我們還建議使用安全手套來保護(hù) 裸露的皮膚。
-板細(xì)胞直接與完整的生長培養(yǎng)基。每1毫升冷凍細(xì)胞使用10至20毫升完整培養(yǎng)基。細(xì)胞接種量應(yīng)至少為3x105細(xì)胞/ml。
-培養(yǎng)細(xì)胞12至24小時(shí)，用新鮮的*培養(yǎng)基替代培養(yǎng)基，去除冷凍保存液。
我們建議解凍程序1，特別是當(dāng)處理敏感細(xì)胞。
預(yù)防措施及免責(zé)聲明
本產(chǎn)品僅供研究用。請(qǐng)查閱材料安全數(shù)據(jù)表，以獲取有關(guān)危險(xiǎn)和安全處理做法的信息。
警告：冷凍Me含有二甲基亞砜(DMSO)。不要吸入氣體/煙霧/蒸汽/噴霧。避免接觸眼睛和皮膚。對(duì)眼睛、呼吸系統(tǒng)和皮膚有刺激性。S-23 S-24/25。
我們生產(chǎn)和測(cè)試了我們的生物加工和細(xì)胞培養(yǎng)血清產(chǎn)品，用于不同的哺乳動(dòng)物細(xì)胞培養(yǎng)應(yīng)用。我們提供各種各樣的特色血清：
液體培養(yǎng)基為所有細(xì)胞培養(yǎng)應(yīng)用提供基本營養(yǎng)。我們的每種高質(zhì)量細(xì)胞培養(yǎng)基都是根據(jù)初公布的配方或?qū)ε囵B(yǎng)基的一致性能和穩(wěn)定性*的修飾而制造的。使用摩羯座培養(yǎng)基是保證細(xì)胞成功生長的宜方法。
我們提供各種產(chǎn)品：
來自摩羯座的試劑和補(bǔ)充劑允許您定制細(xì)胞培養(yǎng)系統(tǒng)的生長條件。所有產(chǎn)品都經(jīng)過嚴(yán)格測(cè)試和設(shè)計(jì)，以提供可重復(fù)性和良性能。
我們提供各種各樣的產(chǎn)品：
Capricorn Scientific產(chǎn)品目錄：
貨號(hào) | 品名 | 規(guī)格 | 價(jià)格 |
DMEM-LPA | DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-LPSTA | DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate | 500 ml | 9.50 € |
DMEM-LPXA | DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-HA | DMEM High Glucose (4.5 g/l), with L-Glutamine | 500 ml | 6.40 € |
DMEM-HHSTA | DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES | 500 ml | 9.50 € |
DMEM-HPA | DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-HPSTA | DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate | 500 ml | 7.00 € |
DMEM-HPXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
DMEM-HXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine | 500 ml | 6.40 € |
DMEM-HXRXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red | 500 ml | 10.50 € |
DMEM-12-A | DMEM/Ham’s F-12, with L-Glutamine | 500 ml | 8.60 € |
DMEM-12-HXA | DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES | 500 ml | 8.60 € |
HAM-12-A | Ham’s F-12, with L-Glutamine | 500 ml | 9.50 € |
IMDM-A | IMDM, with L-Glutamine, without Supplements | 500 ml | 9.00 € |
L15-XA | Leibovitz’s L-15 Medium, w/o L-Glutamine | 500 ml | 12.40 € |
MCC-A | McCoy’s Medium, with L-Glutamine | 500 ml | 12.70 € |
M199-A | Medium 199, with L-Glutamine | 500 ml | 8.90 € |
MEM-A | MEM with Earle’s Salts, with L-Glutamine | 500 ml | 7.20 € |
MEM-STA | MEM with Earle’s Salts, with Stable Glutamine | 500 ml | 9.40 € |
MEM-XA | MEM with Earle’s Salts, w/o L-Glutamine | 500 ml | 6.40 € |
MEMA-RXA | MEM Alpha Modification, w/o L-Glutamine, with Nucleosides | 500 ml | 19.90 € |
MEMA-XRXA | MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides | 500 ml | 13.00 € |
RPMI-A | RPMI 1640, with L-Glutamine | 500 ml | 6.40 € |
RPMI-HA | RPMI 1640, with L-Glutamine, with 25 mM HEPES | 500 ml | 9.90 € |
RPMI-HXA | RPMI 1640, w/o L-Glutamine, with 25 mM HEPES | 500 ml | 9.50 € |
RPMI-STA | RPMI 1640, with Stable Glutamine | 500 ml | 8.40 € |
RPMI-XA | RPMI 1640, w/o L-Glutamine | 500 ml | 5.80 € |
RPMI-XRXA | RPMI 1640, w/o L-Glutamine, w/o Phenol Red | 500 ml | 9.90 € |
FM1-F | FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum | 50 ml | 65.00 € |
FM2-F | FreezeMe Two, Cryopreservation Medium Serum-free | 50 ml | 70.00 € |
TP-A | TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine | 500 ml | 29.90 € |
HP-A | HybridomaPrime, Hybridoma Medium, Serum-free | 500 ml | 34.90 € |
HY-A | HybridomaPlus, Hybridoma Medium, Protein-free | 500 ml | 39.90 € |
FP-A | FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine | 500 ml | 29.90 € |
ERP-B | EndoPrime Complete Medium for C*tion of Primary Cells | 100 ml | on request |
ERP-A | EndoPrime Complete Medium for C*tion of Primary Cells | 500 ml | on request |
HPR-B | HepaPrime Complete Medium for C*tion of Primary Hepatocytes | 100 ml | on request |
HPR-A | HepaPrime Complete Medium for C*tion of Primary Hepatocytes | 500 ml | on request |
NKR-G | NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells | 20 ml | on request |
NKR-B | NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells | 100 ml | on request |
CHOA-500ML | CHO-ONE A | 500 ml | 45.00 € |
CHOE-500ML | CHO-ONE E | 500 ml | 49.00 € |
CHOE-1000ML | CHO-ONE E | 1000ml | 89.00 € |
CHOF1-100ML | CHO-ONE F1 | 100 ml | 23.00 € |
CHOF1-500ML | CHO-ONE F1 | 500 ml | 95.00 € |
CHOF2-10ML | CHO-ONE F2 | 10 ml | 15.00 € |
CHOF2-50ML | CHO-ONE F2 | 50 ml | 49.00 € |
CHO-K1 | CHO-ONE Starter Kit |
| 169.00 € |
CHO-K2 | CHO-ONE Expansion Kit |
| 950.00 € |
DMEM-LPA-P10 | DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 10 L | 14.90 € |
DMEM-LPA-P50 | DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 50 L | 62.90 € |
DMEM-HA-P10 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 15.50 € |
DMEM-HA-P50 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 66.50 € |
DMEM-HPA-P10 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 10 L | 15.50 € |
DMEM-HPA-P50 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 50 L | 66.50 € |
MEM-A-P10 | MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 14.90 € |
MEM-A-P50 | MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 62.90 € |
RPMI-A-P10 | RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 15.50 € |
RPMI-A-P50 | RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 66.50 € |
RPMI-HA-P10 | RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate | 10 L | 20.10 € |
RPMI-HA-P50 | RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate | 50 L | 89.90 € |
FBS-52B | Fetal Bovine Serum, Collected in Australia | 100 ml | on request |
FBS-52A | Fetal Bovine Serum, Collected in Australia | 500 ml | on request |
FBS-12B | Fetal Bovine Serum, Collected in South America | 100 ml | on request |
FBS-12A | Fetal Bovine Serum, Collected in South America | 500 ml | on request |
FBS-22B | Fetal Bovine Serum, Collected in USA | 100 ml | on request |
FBS-22A | Fetal Bovine Serum, Collected in USA | 500 ml | on request |
FBS-HI-12B | Fetal Bovine Serum, Heat Inactivated, Collected in South America | 100 ml | on request |
FBS-HI-12A | Fetal Bovine Serum, Heat Inactivated, Collected in South America | 500 ml | on request |
FBS-HI-22B | Fetal Bovine Serum, Heat Inactivated, Collected in USA | 100 ml | on request |
FBS-HI-22A | Fetal Bovine Serum, Heat Inactivated, Collected in USA | 500 ml | on request |
FBS-11B | Fetal Bovine Serum Advanced, Collected in South America | 100 ml | on request |
FBS-11A | Fetal Bovine Serum Advanced, Collected in South America | 500 ml | on request |
FBS-HI-11B | Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America | 100 ml | on request |
FBS-HI-11A | Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America | 500 ml | on request |
FBS-TET-12B | Fetal Bovine Serum, Tetracycline Negative, Collected in South America | 100 ml | on request |
FBS-TET-12A | Fetal Bovine Serum, Tetracycline Negative, Collected in South America | 500 ml | on request |
FBS-ES-22B | Fetal Bovine Serum, ES cell pre-tested, Collected in USA | 100 ml | on request |
FBS-ES-22A | Fetal Bovine Serum, ES cell pre-tested, Collected in USA | 500 ml | on request |
FBS-DIA-12B | Fetal Bovine Serum, Dialysed, Collected in South America | 100 ml | on request |
FBS-DIA-12A | Fetal Bovine Serum, Dialysed, Collected in South America | 500 ml | on request |
FBS-IG-12B | Fetal Bovine Serum, IgG Stripped, Collected in South America | 100 ml | on request |
FBS-IG-12B | Fetal Bovine Serum, IgG Stripped, Collected in South America | 500 ml | on request |
FBS-ED-12F | Fetal Bovine Serum Exosome Depleted | 50 ml | on request |
FBS-ED-12B | Fetal Bovine Serum Exosome Depleted | 100 ml | on request |
FBS-ED-12A | Fetal Bovine Serum Exosome Depleted | 500 ml | on request |
NCS-1B | Newborn Calf Serum | 100 ml | 12.00 € |
NCS-1A | Newborn Calf Serum | 500 ml | 38.00 € |
NCS-HI-1B | Newborn Calf Serum, Heat Inactivated | 100 ml | 13.10 € |
NCS-HI-1A | Newborn Calf Serum, Heat Inactivated | 500 ml | 43.00 € |
CS-1B | Calf Serum | 100 ml | 8.00 € |
CS-1A | Calf Serum | 500 ml | 29.50 € |
ABS-1B | Adult Bovine Serum | 100 ml | 8.00 € |
ABS-1A | Adult Bovine Serum | 500 ml | 29.50 € |
ABS-1C | Adult Bovine Serum | 1000 ml | on reques |
HOS-1B | Horse Serum | 100 ml | 7.20 € |
HOS-1A | Horse Serum | 500 ml | 32.00 € |
DHS-1B | Donor Horse Serum | 100 ml | 9.00 € |
DHS-1A | Donor Horse Serum | 500 ml | 35.00 € |
GOA-1B | Goat Serum | 100 ml | 24.00 € |
GOA-1A | Goat Serum | 500 ml | 105.00 € |
POR-1B | Porcine Serum | 100 ml | 10.50 € |
POR-1A | Porcine Serum | 500 ml | 39.00 € |
MOU-1B | Mouse Serum | 100 ml | 499.00 € |
RAT-1B | Rat Serum | 100 ml | 499.00 € |
RAB-1B | Rabbit Serum | 100 ml | 99.00 € |
HUM-3B | Human Serum, Type AB, Male | 100 ml | 134.00 € |
BSA-1U | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 50 g | 56.00 € |
BSA-1S | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 100 g | 77.00 € |
BSA-1V | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 250 g | on request |
BSA-1T | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 500 g | 299.00 € |
BSA-FAF-1U | Bovine Serum Albumin, Fatty Acid Free, Origin USA | 50 g | 99.00 € |
BSA-FAF-1S | Bovine Serum Albumin, Fatty Acid Free, Origin USA | 100 g | 178.00 € |
BSA-PF-1U | Bovine Serum Albumin, Protease Free, Origin USA | 50 g | 88.00 € |
BSA-PF-1S | Bovine Serum Albumin, Protease Free, Origin USA | 100 g | 151.00 € |
HSA-1R | Human Serum Albumin | 10 g | 56.00 € |
HSA-1U | Human Serum Albumin | 50 g | 227.00 € |
HSA-1S | Human Serum Albumin | 100 g | 420.00 € |
N2-K | N2 Supplement (100x), Serum-free | 5 ml | 45.00 € |
C21-H | NCS21 Supplement (50x), Serum-free | 10 ml | 45.00 € |
ITS-H | ITS (100x), Insulin, Transferrin, Selenium | 10 ml | 25.00 € |
HCS-F | Hybridoma Supplément, Serum-free | 50 ml | 99.00 € |
GLN-B | L-Glutamine, 200 mM | 100 ml | 8.00 € |
STA-B | Stable Glutamine, 200 mM | 100 ml | 14.50 € |
NPY-B | Sodium Pyruvate, 100 mM | 100 ml | 10.50 € |
NEAA-B | MEM Nonessential Amino Acids (100x) | 100 ml | 8.50 € |
ACC-1B | Accutase Cell Detachment Solution | 100 ml | 26.00 € |
ACM-1G | AccuMax Cell Detachment Solution | 20 ml | 29.00 € |
ACM-1F | AccuMax Cell Detachment Solution | 50 ml | 69.00 € |
TRY-1B | Trypsin-EDTA (0.05 %) in DPBS (1x) | 100 ml | 7.00 € |
TRY-1B10 | Trypsin-EDTA (0.5 %) in DPBS (10x) | 100 ml | 11.50 € |
TRY-2B | Trypsin (0.25 %) in DPBS (1x) | 100 ml | 7.00 € |
TRY-2B10 | Trypsin (2.5 %) in DPBS (10x) | 100 ml | 11.50 € |
TRY-3B | Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red | 100 ml | 9.50 € |
TRY-4B | Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red | 100 ml | 8.50 € |
AMP-B | Amphotericin B Solution (250 µg/ml) | 100 ml | 14.00 € |
AAS-B | Antibiotic/Antimycotic Solution (100x) | 100 ml | 14.00 € |
GEN-10B | Gentamicin Solution (10 mg/ml) | 100 ml | 50.00 € |
PS-B | Penicillin/Streptomycin (100x) | 100 ml | 8.00 € |
PSG-B | Penicillin/Streptomycin (100x), with L-Glutamine | 100 ml | 15.00 € |
MYX-B | MycoXpert, Mycoplasma Removal Reagent (50x) | 100 ml | 99.00 € |
LSM-B | Lymphocytes Separation Media, Density 1.077 g/ml | 100 ml | 12.00 € |
LSM-A | Lymphocytes Separation Media, Density 1.077 g/ml | 500 ml | 36.50 € |
FM1-F | FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum | 50 ml | 65.00 € |
FM2-F | FreezeMe Two, Cryopreservation Medium Serum-free | 50 ml | 70.00 € |
MYX-B | MycoXpert, Mycoplasma Removal Reagent (50x) | 100 ml | 99.00 € |
PBS-10XA | Dulbecco's PBS (10x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 13.00 € |
PBS-1A | Dulbecco's PBS (1x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 6.50 € |
PBS-2A | Dulbecco's PBS (1x), with Ca & Mg, w/o Phenol Red | 500 ml | 8.00 € |
HBSS-1A | Hanks' Balanced Salts (1x), with Ca & Mg, w/o Phenol Red | 500 ml | 7.50 € |
HBSS-2A | Hanks' Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 7.00 € |
HBSS-3A | Hanks' Balanced Salts (1x), w/o Ca & Mg, with Phenol Red | 500 ml | 7.50 € |
HEP-B | HEPES Buffer Solution (1 M) | 100 ml | 39.00 € |
PBS-1A-P10 | Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red | 10 L | 10.90 € |
PBS-1A-P50 | Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red | 50 L | 43.10 € |
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上海起發(fā)實(shí)驗(yàn)試劑有限公司是實(shí)驗(yàn)試劑一站式采購服務(wù)商
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